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authorCalvin Morrison <mutantturkey@gmail.com>2014-01-17 14:20:19 -0500
committerCalvin Morrison <mutantturkey@gmail.com>2014-01-17 14:20:19 -0500
commitecc00aae08c06ae2da5629533ef69d7c5ffd86fe (patch)
tree108d09b652fed106b197f1830e8990b9fa8a5177
parent3bc08d4b610f36887bdd60ecf361f5df75eba944 (diff)
parent4b1c3eead0feece6f25a5293e02f64360f3d5ed1 (diff)
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+SelectiveGenomeAmplification
+============================
+
+PI: http://brisson.bio.upenn.edu/
+
+To use this you'll need:
+
+ - A unix environment
+ - kmer_total_count, a kmer counter available here: http://github.com/mutantturkey/dna-utils/
+ - bash or compliant shell.
+
+
+
+Example Usage:
+
+ cd SelectiveGenomeAmplification;
+ ./selectiveGenomeAmplification.sh PfalciparumGenome.fasta HumanGenome.fasta;
+ less PfalciparumGenome.fasta_HumanGenome_final_mers
+
+For user customizable variables:
+
+ max_mer_distance=5000; max_select=6 min_mer_range=6 max_mer_range=12 \
+ ./SelectiveGenomeAmplification.sh PfalciparumGenome.fasta half.fasta
+
+## Customizable variables
+
+range of mers, min and max
+
+C | variable | default | notes
+:---- | :---- | :---- | ---- | :----
+Y | min_mer_range | 6 | minimum mer size to use
+Y | max_mer_range | 10 | maximum mer size to use
+Y | max_mer_distance | 5000 | maximum distance between mers in foreground
+Y | counts_directory | $PWD/counts | *PWD is current directory
+N | output_directory | $PWD/$foreground_$background/ | ex. if fg is Bacillus.fasta and bg is HumanGenome.fasta then folder would be $PWD/Bacillus_HumanGenome_output/
+Y | tmp_directory=$PWD/tmp | temporary files directory
+Y | max_melting_temp | 30° | maximum melting temp of mers
+Y | min_melting_temp | 0° | minimum melting temp of mers
+Y | min_mer_count | Not Enabled (0) | only select mers that occur more frequently than this number
+Y | max_select | maximum number of mers to pick
+Y | ignore_mers | Not Enabled | mers to explicitly ignore, space seperated ex. ignore_mers="ACAGTA ACCATAA ATATATAT"