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authorCalvin Morrison <mutantturkey@gmail.com>2013-08-30 13:25:46 -0400
committerCalvin Morrison <mutantturkey@gmail.com>2013-08-30 13:25:46 -0400
commit052ac4f46057030e8c489bba29458ee56437cdef (patch)
tree74b24a13d357d7c7b4418d32c60835e9a105ed39
parent50824f178a11861124407840e8b226acf325f86a (diff)
Update cli.markdown
Fix markdown formatting, and add some details about the pre-requisites for multifasta
-rw-r--r--doc/cli.markdown11
1 files changed, 6 insertions, 5 deletions
diff --git a/doc/cli.markdown b/doc/cli.markdown
index e12f6a9..42af4b6 100644
--- a/doc/cli.markdown
+++ b/doc/cli.markdown
@@ -58,7 +58,7 @@ Warning: this program will use a large amount of memory, and CPU time. You can
reduce the number of cores used, and thus memory, by specifying the -j flag
with aspecified number of jobs. Otherwise python with run one job per cpu core.
-# Pre-processing of Multifasta\_to\_otu #
+### Pre-processing of Multifasta\_to\_otu ###
* Please name fasta files of sample reads with <sample id>.fa<*> and place them
into one directory without any other file in that directory (for example, no
@@ -67,7 +67,7 @@ with aspecified number of jobs. Otherwise python with run one job per cpu core.
* Fasta files of reads must have a suffix that starts with .fa (e.g.: .fasta and
.fa are valid while .fna is NOT)
-### Usage ###
+#### Usage ####
multifasta_to_otu's arguments:
-i, --input-directory the directory containing the samples' fasta files of
@@ -92,10 +92,11 @@ with aspecified number of jobs. Otherwise python with run one job per cpu core.
Unweighted.)
Pre-requisites:
-1. <quikr_otu_table.txt>
+
+1. quikr_otu_table.txt (The OTU Table generated with multifasta_to_otu
2. the tree of the database sequences that were used (e.g. dp7\_mafft.fasttree,
gg\_94\_otus\_4feb2011.tre, etc.)
-3. your-defined <qiime_metadata_file.txt>
+3. your-defined qiime_metadata_file.txt ( a Qiime Map file)
The QIIME procedue:
@@ -105,7 +106,7 @@ The QIIME procedue:
make_3d_plots.py -i <quikr_otu_project_name>_weighted.txt -o <3d_pcoa_plotdirectory> -m <qiime_metadata_file>
-#### Broken Pipe Errors ####
+## Broken Pipe Errors ##
Make sure that you have the count-kmers and probablilties-by-read in your
$PATH, and that they are executable.