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// Copyright 2013 Calvin Morrison
#include <errno.h>
#include <stdio.h>
#include <stdlib.h>
#include <string.h>
#include "kmer_total_count.h"
#define ERROR_CODE 5
const unsigned char kmer_alpha[256] =
{5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5,
5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5,
// A C G
5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 0, 5, 1, 5, 5, 5, 2, 5, 5, 5, 5, 5, 5,
// T A C G
5, 5, 5, 5, 5, 5, 3, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 0, 5, 1, 5, 5, 5, 2,
// T
5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 3, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5,
5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5,
5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5,
5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5,
5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5,
5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5};
static const char reverse_alpha[4] = { 'A', 'C', 'G', 'T' };
unsigned long long kmer_pow_four(unsigned long long x) {
return (unsigned long long)1 << (x * 2);
}
// convert a string of k-mer size base-4 values into a
// base-10 index
unsigned long kmer_num_to_index(const char *str, const int kmer, const long error_pos) {
int i = 0;
unsigned long out = 0;
unsigned long multiply = 1;
for(i = kmer - 1; i >= 0; i--){
if(str[i] >> 2) {
return error_pos;
}
out += str[i] * multiply;
multiply = multiply << 2;
}
return out;
}
// convert an index back into a kmer string
char *kmer_index_to_kmer(unsigned long long index, long kmer) {
int i = 0;
int j = 0;
char *num_array = calloc(kmer, sizeof(char));
char *ret = calloc(kmer + 1, sizeof(char));
if(ret == NULL)
exit(EXIT_FAILURE);
// this is the core of the conversion. modulus 4 for base 4 conversion
while (index != 0) {
num_array[i] = index % 4;
index /= 4;
i++;
}
// for our first few nmers, like AAAAA, the output would only be "A" instead
// of AAAAA so we prepend it
for(j = 0; j < (kmer - i); j++)
ret[j] = 'A';
// our offset for how many chars we prepended
int offset = j;
// save i so we can print it
int start = i ;
// decrement i by 1 to reverse the last i++
i--;
j = 0;
// reverse the array, as j increases, decrease i
for(j = 0; j < start; j++, i--)
ret[j + offset] = reverse_alpha[(int)num_array[i]];
// set our last character to the null termination byte
ret[kmer + 1] = '\0';
free(num_array);
return ret;
}
// Strip out any character 'c' from char array 's' into a destination dest (you
// need to allocate that) and copy only len characters.
static char *strnstrip(const char *s, char *dest, int c, unsigned long long len) {
unsigned long long i = 0;
unsigned long long j = 0;
for(i = 0; i < len; i++) {
if(s[i] != c) {
dest[j] = s[i];
j++;
}
}
dest[j] = '\0';
return dest;
}
void translate_nucleotides_to_numbers(char *str, size_t len, const unsigned char *lookup) {
size_t i;
for(i = 0; i < len; ++i) {
str[i] = lookup[(int)str[i]];
}
}
int is_error_char(char c) {
return c == ERROR_CODE;
}
unsigned long long *kmer_counts_from_file(FILE *fh, const unsigned int kmer) {
char *line = NULL;
size_t len = 0;
ssize_t read;
size_t i = 0;
size_t position = 0;
// width is 4^kmer
// there's a sneaky bitshift to avoid pow dependency
const unsigned long width = kmer_pow_four(kmer);
// malloc our return array
unsigned long long *counts = calloc(width + 1, sizeof *counts);
if(counts == NULL) {
fprintf(stderr, strerror(errno));
exit(EXIT_FAILURE);
}
char *str = malloc(4096);
if(str == NULL) {
fprintf(stderr, strerror(errno));
exit(EXIT_FAILURE);
}
unsigned long long str_size = 4096;
while ((read = getdelim(&line, &len, '>', fh)) != -1) {
// find our first \n, this should be the end of the header
char *start = strchr(line, '\n');
if(start == NULL)
continue;
// point to one past that.
start = start + 1;
size_t start_len = strlen(start);
// if our current str buffer isn't big enough, realloc
if(start_len + 1 > str_size + 1) {
str = realloc(str, start_len + 1);
if(str == NULL) {
fprintf(stderr, strerror(errno));
exit(EXIT_FAILURE);
}
}
// strip out all other newlines to handle multiline sequences
str = strnstrip(start, str, '\n',start_len);
size_t seq_length = strlen(str);
// relace A, C, G and T with 0, 1, 2, 3 respectively
// everything else is 5
translate_nucleotides_to_numbers(str, seq_length, kmer_alpha);
// loop through our string to process each k-mer
for(position = 0; position < (seq_length - kmer + 1); position++) {
unsigned long mer = 0;
unsigned long multiply = 1;
// for each char in the k-mer check if it is an error char
for(i = position; i < position + kmer; ++i) {
if(is_error_char(str[i])) {
mer = width;
position = i;
goto next;
}
// multiply this char in the mer by the multiply
// and bitshift the multiply for the next round
mer += str[i] * multiply;
multiply = multiply << 2;
}
// use this point to get mer of our loop
next:
// bump up the mer value in the counts array
counts[mer]++;
}
}
free(line);
free(str);
return counts;
}
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