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import sys
from multiprocessing import Pool
def above_melting_temperature(kmer_with_count):
kmer = kmer_with_count.split("\t")[0]
A = kmer.count('A')
C = kmer.count('C')
G = kmer.count('G')
T = kmer.count('T')
melt_temp = 0.0;
if len(kmer) < 13:
melt_temp = ((A+T) * 2) + ((C+G) * 4)
else:
melt_temp = 64.9 + 41*(G+C-16.4)/(A+T+G+C)
if melt_temp < max_melting_temp:
return kmer_with_count
else:
return 0
lines = sys.stdin.readlines()
max_melting_temp = float(sys.argv[1])
p = Pool()
output = p.map(above_melting_temperature, lines)
for line in output:
if line != 0:
sys.stdout.write(line)
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