1 files changed, 26 insertions, 0 deletions
diff --git a/src/below_melting_temperature.py b/src/below_melting_temperature.py
new file mode 100644
index 0000000..e2521db
--- /dev/null
+++ b/src/below_melting_temperature.py
@@ -0,0 +1,26 @@
+import sys
+
+def in_temp_range(kmer):
+
+	A = kmer.count('A')
+	C = kmer.count('C')
+	G = kmer.count('G')
+	T = kmer.count('T')
+
+	melt_temp = 0.0;
+
+	if len(kmer) < 13:
+		melt_temp = ((A+T) * 2) + ((C+G) * 4)
+	else:
+		melt_temp = 64.9 + 41*(G+C-16.4)/(A+T+G+C)
+
+	return min_melting_temp < melt_temp < max_melting_temp
+
+min_melting_temp = float(sys.argv[1])
+max_melting_temp = float(sys.argv[2])
+
+
+output = []
+for line in sys.stdin:
+	if in_temp_range(line.split("\t")[0]):
+		sys.stdout.write(line)
+\ No newline at end of file