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authormutantturkey <mutantturke@gmail.com>2012-08-03 11:03:31 -0400
committermutantturkey <mutantturke@gmail.com>2012-08-03 11:03:31 -0400
commita8f7aadb1bfbe91d9635e0f57e341729dc2d8360 (patch)
treed8631316482ee54531c392fecd9c9b0cafd92d4b
parentb1cf2ef9c477ac41edc69e99ebcc1e484d48543d (diff)
added more docs
-rw-r--r--README.markdown40
1 files changed, 39 insertions, 1 deletions
diff --git a/README.markdown b/README.markdown
index c49fa40..30ee934 100644
--- a/README.markdown
+++ b/README.markdown
@@ -58,7 +58,7 @@ scripts directory.
1. Cropping and Extracting Cell Images
2. Deriving Cell Backgrounds
3. Generating Cell Masks
-4. Filtering Masks
+4. Filtering Cell Masks
5. Fly Tracking
### Step 1: Cropping and Extracting Cell Images ###
@@ -93,3 +93,41 @@ This will take some time, and require a lot of CPU and disk space.
### Step 2: Deriving Cell Backgrounds ###
+The second step of the process is deriving the background image from the video.
+By doing this, we can subtract the background from the current frame to generate
+the mask. When subtracted the parts left over are the fly, while things in the
+background won't show up.
+
+ process-video-beta -v First10MinGroup1.avi -l Group1First10MinCropFile -n 1 -t First10MinSet -d data/ -2
+
+This will derive the backgrounds for all of the Cells in the video seperately.
+After this tool is done running, it will output the Background.png into the mask
+folder. $data-root/$settype$setnumber/Background.png, for example it might be in
+data/First10MinSet2/Background.png.
+
+Verify this image. Sometimes when the flies do not move the background might
+include the fly! If there are any left of flie parts, use GIMP to airbrush over
+that section with an appropriate color (guess what the background would be
+"close" too).
+
+### Step 3: Generating Cell Masks ###
+
+The third step of the process is generating the Masks from the background and
+the cropped images.
+
+ process-video-beta -v First10MinGroup1.avi -l Group1First10MinCropFile -n 1 -t First10MinSet -d data/ -3
+
+This step will output the masks in $data-root/$settype$setnumber/Masks/
+
+### Step 4: Filtering Cell Masks ###
+
+The fourth step is the filtering step. It's important because it makes sure the
+masks do not have stray objects in them (which will mess up the final tracking
+sequence)
+
+
+ process-video-beta -v First10MinGroup1.avi -l Group1First10MinCropFile -n 1 -t First10MinSet -d data/ -4
+
+The filtered masks are in $data-root/$settype$setnumber/Filtered/final/
+
+