updated documentation , added multi_gg_final.m

2 files changed, 232 insertions, 0 deletions

diff --git a/src/matlab/multifasta2otu/README b/src/matlab/multifasta2otu/README
new file mode 100644
index 0000000..40031e6
--- /dev/null
+++ b/src/matlab/multifasta2otu/README
@@ -0,0 +1,22 @@
+* Please name fasta files of sample reads with <sample id>.fa<*> and place them into one directory without any other file in that directory (for example, no hidden files that the operating system may generate, are allowed in that directory)
+* Note: When making your QIIME Metadata file, the sample id's must match the fasta file prefix names
+* Fasta files of reads must have a suffix that starts with .fa (e.g.: .fasta and .fa are valid while .fna is NOT)
+* Modify the top of the Matlab/Octave scripts for <input_directory>, <output_directory>, <output_filename>, and <training_database_filename>
+
+To use with QIIME, one must run the QIIME conversion tool on our OTU table output:
+convert_biom.py -i <quikr_otu_table.txt> -o <quikr_otu>.biom --biom_table_type="otu table"
+
+---------------------------
+
+4-step QIIME procedure after using Quikr to obtain 3D PCoA graphs:
+(Note: Our code works much better with WEIGHTED Unifrac as opposed to 
+Unweighted.)
+
+Pre-requisites: 1) <quikr_otu_table.txt>, 2) the tree of the database sequences that were used (e.g. 
+rdp7_mafft.fasttree, gg_94_otus_4feb2011.tre, etc.), and 3) your-defined <qiime_metadata_file.txt>
+
+1. convert_biom.py -i <quikr_otu_table.txt> -o <quikr_otu>.biom --biom_table_type="otu table"
+2. beta_diversity.py -i <quikr_otu>.biom -m weighted_unifrac -o beta_div -t <tree file (example: rdp7_mafft.fasttree)>
+3. principal_coordinates.py -i beta_div/weighted_unifrac_<quikr_otu>.txt -o <quikr_otu_project_name>_weighted.txt
+4. make_3d_plots.py -i <quikr_otu_project_name>_weighted.txt -o <3d_pcoa_plotdirectory> -m <qiime_metadata_file>
+
diff --git a/src/matlab/multifasta2otu/multi_gg_final.m b/src/matlab/multifasta2otu/multi_gg_final.m
new file mode 100644
index 0000000..9b66274
--- /dev/null
+++ b/src/matlab/multifasta2otu/multi_gg_final.m
@@ -0,0 +1,210 @@
+%This is an example of how to run Multifasta Quikr with a custom 

+%training database (in this case Greengenes OTU's within 94% identity)

+

+%make sure Matlab/Octave is in your path

+%cd /path/to/Quikr

+

+%User-defined variables

+input_directory='../separated_samples'; %path to input directory of samples

+output_directory='quikr_results'; %path to where want output files to go

+otu_table_name='gg1194_otu_octave.txt'; %name of output otu_table filename

+trainingdatabasefilename='gg_94_otus_4feb2011.fasta'; %full path to the FASTA file you wish to use as a training database

+

+

+mkdir([output_directory]);

+thedirs=dir([input_directory]);

+thetime=zeros(numel(thedirs)-1,1);

+names={};

+

+if(exist('OCTAVE_VERSION')) %check to see if running Octave or Matlab

+

+%This is Octave Version

+

+tic();

+k=6; %pick a k-mer size

+trainingmatrix=quikrTrain(trainingdatabasefilename,k); %this will return the training database

+disp('Training time:')

+[headers,~]=fastaread(trainingdatabasefilename); %read in the training database

+lambda=10000; 

+training_time=toc()

+

+species=struct();

+keys={};

+

+tic();

+

+

+i=0;

+%for numdirs=3:5

+for numdirs=3:numel(thedirs)

+i=i+1;

+disp([num2str(i) ' out of ' num2str(numel(thedirs)-2)])

+fastafilename=[input_directory '/' thedirs(numdirs).name];

+[loadfasta,~]=fastaread(fastafilename);

+numreads=numel(loadfasta);

+xstar=quikrCustomTrained(trainingmatrix,fastafilename,k,lambda);

+

+nonzeroentries=find(xstar); %get the indicies of the sequences quikr predicts are in your sample

+proportionscell=num2cell(xstar(nonzeroentries)); %convert the concentrations into a cell array

+namescell=headers(nonzeroentries); %Get the names of the sequences

+namesandproportions={namescell{:}; proportionscell{:}}; %This cell array contains the (unsorted) names of the reconstructed sequences and their concentrations (in the first and second columns respectively)

+

+[a cols]=size(namesandproportions);

+amount=zeros(cols,1);

+for j=1:cols

+  names{j}=['s' namesandproportions{1,j}];

+  amount(j)=namesandproportions{2,j};

+  if strcmp(keys,names{j})

+	temp=species.(names{j});

+      	temp(i)=round(amount(j).*numreads);

+      	species.(names{j})=temp;

+  else

+      temp=zeros(numel(thedirs)-3+1,1);

+      temp(i)=round(amount(j).*numreads);

+      species.(names{j})=temp;

+      keys{end+1}=names{j};

+  end

+end

+

+thefa=strfind(thedirs(numdirs).name,'.fa');

+

+if ~isempty(thedirs(numdirs).name(1:thefa-1))

+	sampleid{i}=thedirs(numdirs).name(1:thefa-1);

+else

+	sampleid{i}='empty_sampleid';

+end

+

+thetime(i+1)=toc();

+thetime(i+1)

+

+end

+

+disp('Total time to compute Quikr:')

+toc()

+disp('Quickr Average time per file:')

+mean(diff(thetime(1:i+1)))

+

+tic()

+numits=i;

+

+fid=fopen([output_directory '/' otu_table_name],'w');

+fprintf(fid,'# QIIME vGail OTU table\n');

+fprintf(fid,'#OTU_ID\t');

+for i=1:numits

+if i<numits

+fprintf(fid,'%s\t',sampleid{i});

+else

+fprintf(fid,'%s',sampleid{i});

+end

+end

+fprintf(fid,'\n');

+

+for k=1:numel(keys)

+ fprintf(fid,'%s',keys{k}(2:end))

+ temp(:,k)=species.(keys{k});

+        for i=1:numits

+                fprintf(fid,'\t%d',temp(i,k));

+        end

+fprintf(fid,'\n');

+end

+fclose(fid);

+

+disp('Time to output OTU Table:')

+toc()

+

+else

+

+%This is Matlab Version

+

+tic()

+k=6; %pick a k-mer size

+trainingmatrix=quikrTrain(trainingdatabasefilename,k); %this will return the training database

+'Training time:'

+[headers,~]=fastaread(trainingdatabasefilename); %read in the training database

+lambda=10000; 

+training_time=toc()

+

+species=containers.Map;

+

+tic()

+

+

+i=0;

+%for numdirs=3:5

+for numdirs=3:numel(thedirs)

+i=i+1;

+[num2str(i) ' out of ' num2str(numel(thedirs)-2)]

+fastafilename=[input_directory '/' thedirs(numdirs).name];

+[loadfasta,~]=fastaread(fastafilename);

+numreads=numel(loadfasta);

+xstar=quikrCustomTrained(trainingmatrix,fastafilename,k,lambda);

+

+nonzeroentries=find(xstar); %get the indicies of the sequences quikr predicts are in your sample

+proportionscell=num2cell(xstar(nonzeroentries)); %convert the concentrations into a cell array

+namescell=headers(nonzeroentries); %Get the names of the sequences

+namesandproportions={namescell{:}; proportionscell{:}}; %This cell array contains the (unsorted) names of the reconstructed sequences and their concentrations (in the first and second columns respectively)

+

+[a cols]=size(namesandproportions);

+amount=zeros(cols,1);

+for j=1:cols

+  names{j}=namesandproportions{1,j};

+  amount(j)=namesandproportions{2,j};

+  if isKey(species,names{j})

+	 temp=species(names{j});

+      temp(i)=round(amount(j).*numreads);

+      species(names{j})=temp;

+  else

+      temp=zeros(numel(thedirs)-3+1,1);

+      temp(i)=round(amount(j).*numreads);

+      species(names{j})=temp;

+  end

+end

+

+thefa=strfind(thedirs(numdirs).name,'.fa');

+

+if ~isempty(thedirs(numdirs).name(1:thefa-1))

+	sampleid{i}=thedirs(numdirs).name(1:thefa-1);

+else

+	sampleid{i}='empty_sampleid';

+end

+

+thetime(i+1)=toc();

+thetime(i+1)

+

+end

+

+'Total time to compute Quikr:'

+toc()

+'Quickr Average time per file:'

+mean(diff(thetime(1:i+1)))

+

+tic

+numits=i;

+

+fid=fopen([output_directory '/' otu_table_name],'w');

+fprintf(fid,'# QIIME vGail OTU table\n');

+fprintf(fid,'#OTU_ID\t');

+for i=1:numits

+if i<numits

+fprintf(fid,'%s\t',sampleid{i});

+else

+fprintf(fid,'%s',sampleid{i});

+end

+end

+fprintf(fid,'\n');

+

+thekeys=species.keys;

+for k=1:species.Count

+ fprintf(fid,'%s',thekeys{k})

+ temp(:,k)=species(thekeys{k});

+        for i=1:numits

+                fprintf(fid,'\t%d',temp(i,k));

+        end

+fprintf(fid,'\n');

+end

+fclose(fid);

+

+'Time to output OTU Table:'

+toc

+

+end