diff options
author | Calvin Morrison <mutantturkey@gmail.com> | 2014-03-06 17:05:40 -0500 |
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committer | Calvin Morrison <mutantturkey@gmail.com> | 2014-03-06 17:05:40 -0500 |
commit | 2c038ba630c14c7030186c64e9eb92761ddcba74 (patch) | |
tree | f4705db1e2603bdc831254eee60800eb10448fcf /kmer_continuous_count.c | |
parent | 5d7e67a846ec104da2d7bdb988672fbd02ddda28 (diff) |
add kmer_continuous_count
this tool will count continuously, instead of line by line. The way that
this works out is something like this:
test.fa
> header 1
AAAAATTTTT
> header 2
GGGGGAAAAA
counting 6 mers, the program will count TTTGGG, TTGGGG, TGGGGG, like
there was no header seperating them. This can be useful for certain
tyeps of processing, like when the sequences are continuous from a
genome.
initial commit
Diffstat (limited to 'kmer_continuous_count.c')
-rw-r--r-- | kmer_continuous_count.c | 158 |
1 files changed, 158 insertions, 0 deletions
diff --git a/kmer_continuous_count.c b/kmer_continuous_count.c new file mode 100644 index 0000000..5ace9a5 --- /dev/null +++ b/kmer_continuous_count.c @@ -0,0 +1,158 @@ + +// Copyright 2013 Calvin Morrison +#include <errno.h> +#include <stdbool.h> +#include <stdio.h> +#include <stdlib.h> +#include <string.h> +#include <getopt.h> + +#include "kmer_utils.h" + + +void help() { + printf("usage: kmer_total_count -i input_file -k kmer [-n] [-l] ...\n\n" + "count mers in size k from a fasta file, but do so continuously\n" + "\n" + " --input -i input fasta file to count\n" + " --kmer -k size of mers to count\n" + " --nonzero -n only print non-zero values\n" + " --label -l print mer along with value\n" + "\n" + "Report all bugs to mutantturkey@gmail.com\n" + "\n" + "Copyright 2014 Calvin Morrison, Drexel University.\n" + "\n" + "If you are using any dna-utils tool for a publication\n" + "please cite your usage:\n\n" + "dna-utils. Drexel University, Philadelphia USA, 2014;\n" + "software available at www.github.com/EESI/dna-utils/\n"); +} + + +int main(int argc, char **argv) { + + char *fn = NULL; + FILE *fh = NULL; + + unsigned int kmer = 0; + + bool nonzero = false; + bool label = false; + bool kmer_set = false; + + unsigned long long width = 0; + + unsigned long long i = 0; + + static struct option long_options[] = { + {"input", required_argument, 0, 'i'}, + {"kmer", required_argument, 0, 'k'}, + {"nonzero", no_argument, 0, 'n'}, + {"label", no_argument, 0, 'l'}, + {"help", no_argument, 0, 'h'}, + {0, 0, 0, 0} + }; + + while (1) { + + int option_index = 0; + int c = 0; + + c = getopt_long (argc, argv, "i:k:nlvh", long_options, &option_index); + + if (c == -1) + break; + + switch (c) { + case 'i': + fn = optarg; + break; + case 'k': + kmer = atoi(optarg); + kmer_set = true; + break; + case 'n': + nonzero = true; + break; + case 'l': + label = true; + break; + case 'h': + help(); + exit(EXIT_SUCCESS); + break; + case 'v': + printf("dna-utils version " VERSION "\n"); + exit(EXIT_SUCCESS); + break; + default: + break; + } + } + if(argc == 1) { + help(); + exit(EXIT_FAILURE); + } + if(fn == NULL) { + fprintf(stderr, "no input file specified with -i, reading from stdin\n"); + fh = stdin; + } + else { + fh = fopen(fn, "r"); + if(fh == NULL) { + fprintf(stderr, "Could not open %s - %s\n", fn, strerror(errno)); + exit(EXIT_FAILURE); + } + } + if(!kmer_set) { + fprintf(stderr, "Error: kmer (-k) must be supplied\n"); + exit(EXIT_FAILURE); + } + if(kmer == 0) { + fprintf(stderr, "Error: invalid kmer - '%d'.\n", kmer); + exit(EXIT_FAILURE); + } + + width = pow_four(kmer); + + unsigned long long *counts = get_continuous_kmer_counts_from_file(fh, kmer); + + // If nonzero is set, only print non zeros + if(nonzero) { + // if labels is set, print out our labels + if(label) { + for(i = 0; i < width; i++) + if(counts[i] != 0) { + char *kmer_str = index_to_kmer(i, kmer); + fprintf(stdout, "%s\t%llu\n", kmer_str, counts[i]); + free(kmer_str); + } + + } + else { + for(i = 0; i < width; i++) + if(counts[i] != 0) + fprintf(stdout, "%llu\t%llu\n", i, counts[i]); + + } + } + // If we aren't printing nonzeros print everything + else { + if(label) { + for(i = 0; i < width; i++) { + char *kmer_str = index_to_kmer(i, kmer); + fprintf(stdout, "%s\t%llu\n", kmer_str, counts[i]); + free(kmer_str); + } + } + else { + for(i = 0; i < width; i=i+4) { + fprintf(stdout, "%llu\n%llu\n%llu\n%llu\n", counts[i], counts[i+1], counts[i+2], counts[i+3]); + } + } + } + + free(counts); + return EXIT_SUCCESS; +} |