#include #include #include #include #include #include #include #include #include #include "kmer_utils.h" #include "quikr_functions.h" #define USAGE "Usage:\n\tquikr_train [OPTION...] - train a database for use with quikr.\n\nOptions:\n\n-i, --input\n\tthe database of sequences to create the sensing matrix (fasta format)\n\n-k, --kmer\n\tspecify what size of kmer to use. (default value is 6)\n\n-o, --output\n\tthe sensing matrix. (a gzip'd text file)\n\n-v, --verbose\n\tverbose mode.\n\n-V, --version\n\tprint version." int main(int argc, char **argv) { // getline variables char *line = NULL; size_t len = 0; ssize_t read; int c; // k-mer is 6 by default int kmer = 6; // revision number int revision = 0; // iterators long long i = 0; long long position = 0; int verbose = 0; char *fasta_filename = NULL; char *output_file = NULL; gzFile output = NULL; FILE *input = NULL; while (1) { static struct option long_options[] = { {"verbose", no_argument, 0, 'v'}, {"help", no_argument, 0, 'h'}, {"version", no_argument, 0, 'V'}, {"input", required_argument, 0, 'i'}, {"kmer", required_argument, 0, 'k'}, {"output", required_argument, 0, 'o'}, {0, 0, 0, 0} }; int option_index = 0; c = getopt_long (argc, argv, "i:o:k:hvV", long_options, &option_index); if (c == -1) break; switch (c) { case 'i': fasta_filename = optarg; break; case 'k': kmer = atoi(optarg); break; case 'o': output_file = optarg; break; case 'v': verbose = 1; break; case 'V': printf("%s\n", VERSION); exit(EXIT_SUCCESS); break; case 'h': printf("%s\n", USAGE); exit(EXIT_SUCCESS); break; case '?': /* getopt_long already printed an error message. */ break; default: exit(EXIT_FAILURE); } } if(fasta_filename == NULL) { fprintf(stderr, "Error: input fasta file (-i) must be specified\n\n"); fprintf(stderr, "%s\n", USAGE); exit(EXIT_FAILURE); } if(output_file == NULL) { fprintf(stderr, "Error: output matrix file (-o) must be specified\n\n"); fprintf(stderr, "%s\n", USAGE); exit(EXIT_FAILURE); } if(verbose) { printf("kmer size: %d\n", kmer); printf("fasta file: %s\n", fasta_filename); printf("output file: %s\n", output_file); } if(access (fasta_filename, F_OK) == -1) { fprintf(stderr, "Error: could not find %s\n", fasta_filename); exit(EXIT_FAILURE); } if(kmer == 0) { fprintf(stderr, "Error: zero is not a valid kmer\n"); exit(EXIT_FAILURE); } if(strcmp(&output_file[strlen(output_file) - 3], ".gz") != 0) { char *temp = malloc(strlen(output_file) + 4); if(temp == NULL) { fprintf(stderr, "Could not allocate enough memory\n"); exit(EXIT_FAILURE); } sprintf(temp, "%s.gz", output_file); output_file = temp; printf("appending a .gz to our output file: %s\n", output_file); } // 4 ^ Kmer gives us the width, or the number of permutations of ACTG with // kmer length long width = pow(4, kmer); unsigned long sequences = count_sequences(fasta_filename); if(sequences == 0) { fprintf(stderr, "Error: %s contains 0 fasta sequences\n", fasta_filename); } if(verbose) { printf("sequences: %ld\nwidth: %ld\n", sequences, width); printf("Writing our sensing matrix to %s\n", output_file); } input = fopen(fasta_filename, "r" ); if(input == NULL) { fprintf(stderr, "Error opening %s - %s\n", fasta_filename, strerror(errno)); exit(EXIT_FAILURE); } // open our output file output = gzopen(output_file, "w"); if(output == NULL) { fprintf(stderr, "Error: could not open output file, error code: %s\n", strerror(errno)); exit(EXIT_FAILURE); } // create our header gzprintf(output, "quikr\n"); gzprintf(output, "%ld\n", revision); gzprintf(output, "%ld\n", sequences); gzprintf(output, "%d\n", kmer); // malloc our return array unsigned long long * counts = malloc((width + 1) * sizeof(unsigned long long)); if(counts == NULL) { fprintf(stderr, strerror(errno)); exit(EXIT_FAILURE); } char *str = malloc(4096); if(str == NULL) { fprintf(stderr, strerror(errno)); exit(EXIT_FAILURE); } unsigned long long str_size = 4096; // seek the first character, and skip over it fseek(input, 1, SEEK_CUR); while ((read = getdelim(&line, &len, '>', input)) != -1) { // find first whitespace for(i = 0; i < read; i ++) { if(line[i] == ' ' || line[i] == '\t' || line[i] == '\n') break; } // write our header gzprintf(output, ">%.*s\n", i, line); // find our first \n, this should be the end of the header char *start = strchr(line, '\n'); if(start == NULL) continue; size_t start_len = strlen(start); // if our current str buffer isn't big enough, realloc if(start_len + 1 > str_size + 1) { str = realloc(str, start_len + 1); if(str == NULL) { exit(EXIT_FAILURE); fprintf(stderr, strerror(errno)); } } // strip out all other newlines to handle multiline sequences str = strnstrip(start, str, '\n',start_len); size_t seq_length = strlen(str); // relace A, C, G and T with 0, 1, 2, 3 respectively // everything else is 5 for(i = 0; i < seq_length; i++) { str[i] = alpha[(int)str[i]]; } // set counts to zero memset(counts, 0, width * sizeof(counts)); // loop through our string to process each k-mer for(position = 0; position < (seq_length - kmer + 1); position++) { unsigned long mer = 0; unsigned long multiply = 1; // for each char in the k-mer check if it is an error char for(i = position + kmer - 1; i >= position; i--){ if(str[i] >> 2) { mer = width; position = i; goto next; } // multiply this char in the mer by the multiply // and bitshift the multiply for the next round mer += str[i] * multiply; multiply = multiply << 2; } // use this point to get mer of our loop next: // bump up the mer value in the counts array counts[mer]++; } for(i = 0; i < width; i++) { gzprintf(output, "%lld\n", counts[i]); } } free(counts); free(line); gzclose(output); fclose(input); return EXIT_SUCCESS; }