#!/usr/bin/python import numpy as np import os import sys import gzip from subprocess import * import platform import argparse def main(): """ You can call this script independently, and will save the trained matrix as a numpy file. example: python quikr-train.py -i input.fasta -k 6 -o trained_matrix.npy """ parser = argparse.ArgumentParser(description= " quikr_train returns a custom trained matrix that can be used with \ the quikr function. \n You must supply a kmer. \n ") parser.add_argument("-i", "--input", help="training database of sequences (fasta format)", required=True) parser.add_argument("-o", "--output", help="sensing matrix (text file)", required=True) parser.add_argument("-k", "--kmer", help="kmer size (integer)", type=int, required=False ) parser.add_argument("-z", "--compress", help="compress output (integer)", action='store_true', required=False) args = parser.parse_args() if not os.path.isfile(args.input): parser.error( "Input database not found") # call the quikr train function, save the output with np.save matrix = quikr_train(args.input, args.kmer) if args.compress: output_file = gzip.open(args.output, "wb") else: output_file = open(args.output, "wb") np.save(output_file, matrix) return 0 def quikr_train(input_file_location, kmer): """ Takes a input fasta file, and kmer, returns a custom trained matrix """ kmer_file_name = str(kmer) + "mers.txt" if not os.path.isfile(kmer_file_name): print "could not find kmer file" exit() uname = platform.uname()[0] if uname == "Linux": input_file = Popen(["./probabilities-by-read-linux", str(kmer), input_file_location, kmer_file_name], stdout=PIPE) elif uname == "Darwin": input_file = Popen(["./probabilities-by-read-osx", str(kmer), input_file_location, kmer_file_name]) # load and normalize the matrix by dividing each element by the sum of it's column. # also do some fancy rotations so that it works properly with quikr matrix = np.loadtxt(input_file.stdout) matrix = np.rot90(matrix) matrix = matrix / matrix.sum(0) matrix = np.flipud(matrix); return matrix if __name__ == "__main__": sys.exit(main())