#!/usr/bin/python from multiprocessing import Pool import multiprocessing import os import quikr_train as qt import quikr as q import sys import numpy as np import argparse import platform # our defaults kmer = 6 lamb = 10000 trained_matrix = "" output_directory = "" input_directory = "" def main(): global kmer global input_directory global output_directory global lamb global trained_matrix #do: write up the description parser = argparse.ArgumentParser(description="MultifastaOTU") parser.add_argument("-i", "--input-directory", help="directory containing fasta files", required=True) parser.add_argument("-o", "--otu-table", help="otu_table", required=True) parser.add_argument("-t", "--trained-matrix", help="otu_table", required=True) parser.add_argument("-d", "--output-directory", help="quikr output directory", required=True) parser.add_argument("-l", "--lamb", type=int, help="the default lambda value is 10,000") parser.add_argument("-k", "--kmer", type=int, help="specifies which kmer to use, default=6") parser.add_argument("-j", "--jobs", type=int, help="specifies how many jobs to run at once, default=number of CPUs") args = parser.parse_args() # our defaults jobs=multiprocessing.cpu_count() trained_matrix = args.trained_matrix input_directory = args.input_directory output_directory = args.output_directory # Make sure our input exist if not os.path.isdir(args.input_directory): parser.error( "Input directory not found") if not os.path.isdir(args.output_directory): parser.error( "Input directory not found") if not os.path.isdir(args.output_directory): os.path.mkdir(args,output_directory) if not os.path.isfile(args.trained_matrix): parser.error("custom trained matrix not found") # use alternative lambda if args.lamb is not None: lamb = args.lamb if args.jobs is not None: jobs = args.jobs if args.kmer is not None: kmer = args.kmer fasta_list = os.listdir(args.input_directory) pool = Pool(processes=jobs) result = pool.map(quikr_call, fasta_list) return 0 def quikr_call(fasta_file): inp = input_directory + fasta_file output = output_directory + os.path.basename(fasta_file) xstar = q.quikr(inp, trained_matrix, kmer, lamb) np.savetxt(output, xstar, delimiter=",", fmt="%f") return 0 if __name__ == "__main__": sys.exit(main())