From 4ca6f92ceb4b2f8c504431cf56f8a6135187a61c Mon Sep 17 00:00:00 2001 From: Calvin Date: Mon, 11 Mar 2013 12:36:42 -0400 Subject: adding matlab code --- src/matlab/Example.m | 52 +++++++++ src/matlab/fastaread.m | 232 ++++++++++++++++++++++++++++++++++++++++ src/matlab/quikr.m | 52 +++++++++ src/matlab/quikrCustomTrained.m | 42 ++++++++ src/matlab/quikrTrain.m | 41 +++++++ src/matlab/quikrWriteOutput.m | 89 +++++++++++++++ 6 files changed, 508 insertions(+) create mode 100644 src/matlab/Example.m create mode 100644 src/matlab/fastaread.m create mode 100644 src/matlab/quikr.m create mode 100644 src/matlab/quikrCustomTrained.m create mode 100644 src/matlab/quikrTrain.m create mode 100644 src/matlab/quikrWriteOutput.m (limited to 'src') diff --git a/src/matlab/Example.m b/src/matlab/Example.m new file mode 100644 index 0000000..d2a3192 --- /dev/null +++ b/src/matlab/Example.m @@ -0,0 +1,52 @@ +% Here is an example of re-training the quikr method, performing the reconstruction, and outputing the results to a CSV file appropriate for Excel. +mat=quikrTrain('TaxCollectortrainset7_112011.fa',6); %retrain using the TaxCollector version of trainset7_112011.fa +xstar=quikrCustomTrained(mat,'testfastafile',6,10000); %perform the reconstruction +quikrWriteOutput('/path/to/output/director','filenameforoutput',full(xstar),'/path/to/TaxCollectortrainset7_112011.fa',2) %write the output in CSV format, the last argument (2) corresponds to the phylum level. See the file quikrWriteOutput.m for more details. + + + + +%This is an example of how to run Quikr + +%If you want to use the default training-set (that is, trainset7_112011.fa +%from RPD), then execute the following + +%make sure Matlab/Octave is in the Quikr director +cd /path/to/Quikr + +fastafilename='/path/to/your/fastafile.fasta'; %full path name to your data file +xstar=quikr(fastafilename); %this will give the predicted reconstruction frequencies + + +%xstar will be on the same basis as trainset7_112011.fa, so to get the +%sequences that are predicted to be present in your sample: +[headers,~]=fastaread('trainset7_112011.fa'); %read in the training database +%note fastaread is not by default included in Octave, the fastaread.m file +%is included in the Quikr download directory however, and is directly +%compatible with Matlab. +nonzeroentries=find(xstar); %get the indicies of the sequences quikr predicts are in your sample +proportionscell=num2cell(xstar(nonzeroentries)); %convert the concentrations into a cell array +namescell=headers(nonzeroentries); %Get the names of the sequences +namesandproportions={namescell{:}; proportionscell{:}}; %This cell array contains the (unsorted) names of the reconstructed sequences and their concentrations (in the first and second columns respectively) +%so to find which sequence is the most abundant in your mixture: +[val,ind]=max(xstar(nonzeroentries)); %get the maximum value and it's position +namesandproportions{1:2,ind} %note that this does not imply this specific strain or species is in your sample, just that phylum/class/order/family/genus this species belongs to is in your sample. + + +% If you would like to use a custom training database, follow the following +% steps +fastafilename='/path/to/your/fastafile.fasta'; %full path name to your data file +trainingdatabasefilename='/path/to/your/trainingdatabase.fasta'; %full path to the FASTA file you wish to use as a training database +k=6; %pick a k-mer size +trainingmatrix=quikrTrain(trainingdatabasefilename,k); %this will return the training database +%then to do the reconstuction +lambda=10000; %pick a lambda (larger lambda -> theoretically predicted concentrations are closer to actual concentrations), this depends on k-mer size picked, also size and condition of the TrainingMatrix +xstar=quikrCustomTrained(trainingmatrix,fastafilename,k,lambda); %get the predicted reconstruction frequencies + +%again Xstar is on the same basis as the TrainingMatrix, so to get the +%sequences that are predicted to be present in your sample: +[headers,~]=fastaread(trainingdatabasefilename); %read in the training database +nonzeroentries=find(xstar); %get the indicies of the sequences quikr predicts are in your sample +proportionscell=num2cell(xstar(nonzeroentries)); %convert the concentrations into a cell array +namescell=headers(nonzeroentries); %Get the names of the sequences +namesandproportions={namescell{:}; proportionscell{:}}; %This cell array contains the (unsorted) names of the reconstructed sequences and their concentrations (in the first and second columns respectively) diff --git a/src/matlab/fastaread.m b/src/matlab/fastaread.m new file mode 100644 index 0000000..37d9b71 --- /dev/null +++ b/src/matlab/fastaread.m @@ -0,0 +1,232 @@ +function [data, seq] = fastaread(filename,varargin) +%FASTAREAD reads FASTA format file. +% +% S = FASTAREAD(FILENAME) reads a FASTA format file FILENAME, returning +% the data in the file as a structure. FILENAME can also be a URL or +% MATLAB character array that contains the text of a FASTA format file. +% S.Header is the header information. S.Sequence is the sequence stored +% as a string of characters. +% +% [HEADER, SEQ] = FASTAREAD(FILENAME) reads the file into separate +% variables HEADER and SEQ. If the file contains more than one sequence, +% then HEADER and SEQ are cell arrays of header and sequence information. +% +% FASTAREAD(...,'IGNOREGAPS',TF) removes any gap symbol ('-' or '.') +% from the sequence(s) when TF is true. Default is false. +% +% FASTAREAD(...,'BLOCKREAD', M) allows you to read in a single entry or +% block of entries from a file containing multiple sequences. If M is a +% scalar then the M'th entry in the file is read. If M is a two element +% vector then the block of entries starting at entry M(1) and ending at +% entry M(2) will be read. Use Inf for M(2) to read all entries in the +% file starting at position M(1). +% +% FASTAREAD(...,'TRIMHEADERS',TF) trims the header after the first +% whitespace when TF is true. White space characters include a horizontal +% tab (char(9)) and a space (char(32)). Default is false. +% +% FASTA format specified here: +% http://www.ncbi.nlm.nih.gov/BLAST/fasta.shtml +% +% Examples: +% +% % Read the sequence for the human p53 tumor gene. +% p53nt = fastaread('p53nt.txt') +% +% % Read the sequence for the human p53 tumor protein. +% p53aa = fastaread('p53aa.txt') +% +% % Read a block of entries from a file +% pf2_5_10 = fastaread('pf00002.fa','blockread',[ 5 10], ... +% 'ignoregaps',true) +% +% % Read the human mitochondrion genome in FASTA format. +% entrezSite = 'http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?' +% textOptions = '&txt=on&view=fasta' +% genbankID = '&list_uids=NC_012920' +% mitochondrion = fastaread([entrezSite textOptions genbankID]) +% +% See also EMBLREAD, FASTAINFO, FASTAWRITE, FASTQINFO, FASTQREAD, +% FASTQWRITE, GENBANKREAD, GENPEPTREAD, HMMPROFDEMO, MULTIALIGNREAD, +% MULTIALIGNVIEWER, SEQPROFILE, SEQTOOL, SFFINFO, SFFREAD. + +% Copyright 2002-2010 The MathWorks, Inc. +% $Revision: 1.15.4.21 $ $Date: 2010/06/26 04:54:38 $ + +% check input is char +% in a future version we may accept also cells +if ~ischar(filename) + error('Bioinfo:fastaread:InvalidInput','Input must be a character array') +end + +% default +ignoreGaps = false; +trimh = false; +blockRead = false; +% get input arguments +if nargin > 1 + if rem(nargin,2) ~= 1 + error('Bioinfo:fastaread:IncorrectNumberOfArguments',... + 'Incorrect number of arguments to %s.',mfilename); + end + okargs = {'ignoregaps','blockread','trimheaders'}; + for j=1:2:nargin-1 + pname = varargin{j}; + pval = varargin{j+1}; + k = find(strncmpi(pname,okargs,numel(pname))); + if isempty(k) + error('Bioinfo:fastaread:UnknownParameterName',... + 'Unknown parameter name: %s.',pname); + %elseif length(k)>1 + % error('Bioinfo:fastaread:AmbiguousParameterName',... + % 'Ambiguous parameter name: %s.',pname); + else + switch(k) + case 1 % ignore gaps + ignoreGaps = opttf(pval); + if isempty(ignoreGaps) + error('Bioinfo:fastaread:IgnoreGapsNotLogical',... + '%s must be a logical value, true or false.',... + upper(char(okargs(k)))); + end + case 2 % range + range = pval; + if ~isnumeric(range) || numel(range)> 2 || isempty(range) + error('Bioinfo:fastaread:BadBlockRange',... + 'BlockRange must be numeric scalar or vector with two values.') + end + blockRead = true; + range = sort(range); + case 3 % trimheaders + trimh = opttf(pval, okargs{k}, mfilename); + end + end + end +end + + +if size(filename,1)>1 % is padded string + if blockRead + warning('Bioinfo:fastaread:IgnoredRange',... + 'BLOCKREAD is only allowed when parsing a file.') + end + ftext = cell(size(filename,1),1); + for i=1:size(filename,1) + ftext(i,1)=strtrim(strread(filename(i,:),'%s','whitespace','','delimiter','\n')); + end + % try then if it is an url +elseif (strfind(filename(1:min(10,end)), '://')) + if blockRead + warning('Bioinfo:fastaread:IgnoredRange',... + 'BLOCKREAD is only allowed when parsing a file.') + end + if (~usejava('jvm')) + error('Bioinfo:fastaread:NoJava',... + 'Reading from a URL requires Java.') + end + try + ftext = urlread(filename); + catch allExceptions + error('Bioinfo:fastaread:CannotReadURL',... + 'Cannot read URL "%s".', filename); + end + ftext = strread(ftext,'%s','delimiter','\n'); + + % try then if it is a valid filename +elseif (exist(filename,'file') || exist(fullfile(pwd,filename),'file')) + if blockRead + blockText = getfileblock(filename,range,'>'); + try + ftext = strread(blockText,'%s','delimiter','\n'); + catch theErr + if strcmpi(theErr.identifier,'MATLAB:nomem') + error( 'Bioinfo:fastaread:BlockTooBig',... + 'The block is too large to fit in memory. Try a smaller range.'); + else + rethrow(theErr); + end + end + else %=== read entire file + fid = fopen(filename); + c = onCleanup(@()fclose(fid)); + try + ftext = textscan(fid,'%s','delimiter','\n','bufsize',10000000); + ftext = ftext{:}; + catch theErr + if strcmpi(theErr.identifier,'MATLAB:nomem') + error( 'Bioinfo:fastaread:FileTooBig',... + 'The file is too large to fit in memory. Try using the BLOCKREAD option to read the file in blocks.'); + else + rethrow(theErr); + end + end + end +else % must be a string with '\n', convert to cell + if blockRead + warning('Bioinfo:fastaread:IgnoredRange',... + 'BLOCKREAD is only allowed when parsing a file.') + end + ftext = strread(filename,'%s','delimiter','\n'); +end + +% it is possible that there will be multiple sequences +commentLines = strncmp(ftext,'>',1); + +if ~any(commentLines) + error('Bioinfo:fastaread:FastaNotValid',... + 'Input does not exist or is not a valid FASTA file.') +end + +numSeqs = sum(commentLines); +seqStarts = [find(commentLines); size(ftext,1)+1]; +data(numSeqs,1).Header = ''; + +try + for theSeq = 1:numSeqs + % Check for > symbol ? + data(theSeq).Header = ftext{seqStarts(theSeq)}(2:end); + firstRow = seqStarts(theSeq)+1; + lastRow = seqStarts(theSeq+1)-1; + numChars = cellfun('length',ftext(firstRow:lastRow)); + numSymbols = sum(numChars); + data(theSeq).Sequence = repmat(' ',1,numSymbols); + pos = 1; + for i=firstRow:lastRow, + str = strtrim(ftext{i}); + len = length(str); + if len == 0 + break + end + data(theSeq).Sequence(pos:pos+len-1) = str; + pos = pos+len; + end + data(theSeq).Sequence = strtrim(data(theSeq).Sequence); + if ignoreGaps + data(theSeq).Sequence = strrep(data(theSeq).Sequence,'-',''); + data(theSeq).Sequence = strrep(data(theSeq).Sequence,'.',''); + end + end + + % trim headers + if trimh + for i = 1:numSeqs + data(i).Header = sscanf(data(i).Header,'%s',1); + end + end + + % in case of two outputs + if nargout == 2 + if numSeqs == 1 + seq = data.Sequence; + data = data.Header; + else + seq = {data(:).Sequence}; + data = {data(:).Header}; + end + end + +catch allExceptions + error('Bioinfo:fastaread:IncorrectDataFormat',... + 'Incorrect data format in fasta file') +end + diff --git a/src/matlab/quikr.m b/src/matlab/quikr.m new file mode 100644 index 0000000..d24596e --- /dev/null +++ b/src/matlab/quikr.m @@ -0,0 +1,52 @@ +function xstar = quikr(inputfasta) +%xstar=quikr('path/to/input/fasta/file') returns the estimated frequencies +%of bacteria present when given an input FASTA file of amplicon (454) +%reads. A k-mer based, L1-regularized, sparsity promoting algorithm is +%utilized. This is the default implementation of Quikr, here lambda=10,000, k-mer +%size is 6, and we use the trainset7_112011 database from RDP to estimate +%the present species. In practice, reconstruction is accurate only down to +%the genus level (not species or strain). +%The program ./count-linux or ./count-osx needs to be placed in the same +%directory as this script. In Linux, you might need to: chmod a+rx count. +%Also the inputfasta needs to be a standard FASTA file. The input FASTA file must have reads all in the same +%orientation, so as a preprocessing step, make sure everything is in the +%forward (+) orientation. +if nargin>1 + error('too many input arguments'); +end +if (isunix && not(ismac)) %If linux, use ./count-linux + [status, counts]=unix(['./count-linux -r 6 -1 -u ' inputfasta]); %count the 6-mers in the fasta file, in the forward direction, return the counts without labels + if status ~= 0 + error('./count-linux failed: ensure count-linux is an executable. Try chmod a+rx count-linux. Be sure matlab/octave is in the same directory as count-linux'); + end +elseif ismac %If mac, then use ./count-osx + [status, counts]=unix(['./count-osx -r 6 -1 -u ' inputfasta]); %count the 6-mers in the fasta file, in the forward direction, return the counts without labels + if status ~= 0 + error('./count-osx failed: ensure count-linux is an executable. Try chmod a+rx count-osx. Be sure matlab/octave is in the same directory as count-osx'); + end +elseif ispc + error('Windows is not yet supported'); +end +counts=textscan(counts,'%f'); %convert into floats +counts=counts{:}; %make into a vector +counts=counts/sum(counts); %form a probability vector from the counts +lambda=10000; %this is the default lambda value (see the paper), and is used in the formation of trainset7_112011N6Aaux.mat +yaux=[0;lambda*counts]; % form the sample vector +load('trainset7_112011N6Aaux.mat') %load the 6-mer sensing matrix...I need to rename this so the variable is just Aaux. +if not(exist('Aaux2')) %make sure Aaux2 loaded + error('The default training database trainset7_112011N6Aaux.mat failed to load. Be sure it exists in the Quikr folder or current directory'); +end +%based on RDP's trainset 7 from 11/2011 +isOctave = exist('OCTAVE_VERSION') ~= 0; %check to see if running Octave or Matlab +if isOctave %if octave, need to manually read in sparse-matrix format + n=length(Aaux2.jc)-1; + ii=repelems(1:n,[1:n; diff(Aaux2.jc)]); + Aaux=sparse(Aaux2.ir+1,ii,Aaux2.data); +else + Aaux=Aaux2; %fix the names + clear Aaux2; +end %Matlab automatically reads it in correctly, just need to rename it +warning off +xstar=lsqnonneg(Aaux,yaux); %perform the nonnegative least squares +warning on +xstar=xstar/sum(xstar); %transform the output into a probability vector. Note this vector is on the same basis as Aaux, so the entries correspond to sequences in trainset7_112011.fa \ No newline at end of file diff --git a/src/matlab/quikrCustomTrained.m b/src/matlab/quikrCustomTrained.m new file mode 100644 index 0000000..b58aa31 --- /dev/null +++ b/src/matlab/quikrCustomTrained.m @@ -0,0 +1,42 @@ +function xstar = quikrCustomTrained(trainingmatrix,inputfasta,k,lambda) +%xstar=quikrCustomTrained(traininmatrix,inputfasta,k,lambda) is the +%implementation of qukir that lets you use a custom training matrix +%"trainingmatrix" on the input data file "inputfasta" for the k-mer size +%"k" (note this k-mer size must be such that the number of rows of +%"trainingmatrix"=4^k), with the regularization value "lambda". The vector +%of predicted concentrations "xstar" is returned on the same basis as +%"trainingmatrix". +if nargin~=4 + error('There must be exactly 4 input arguments: the training matrix, the /path/to/input/fastafile, the k-mer size, and lambda'); +end + +[rws, clumns]=size(trainingmatrix); %get the size of the training matrix +if rws~=4^k + error('Wrong k-mer size for input training matrix'); +end + +if (isunix && not(ismac)) + [status, counts]=unix([sprintf('./count-linux -r %d -1 -u ',k) ' ' inputfasta]); %count the 6-mers in the fasta file, in the forward direction, return the counts without labels. + if status ~= 0 + error('./count-linux failed: ensure count-linux is an executable. Try chmod a+rx count-linux. Be sure matlab/octave is in the same directory as count-linux'); + end +elseif ismac + [status, counts]=unix([sprintf('./count-osx -r %d -1 -u ',k) ' ' inputfasta]); %count the 6-mers in the fasta file, in the forward direction, return the counts without labels. + if status ~= 0 + error('./count-osx failed: ensure count-linux is an executable. Try chmod a+rx count-osx. Be sure matlab/octave is in the same directory as count-osx'); + end +elseif ispc + error('Windows is not yet supported'); +end + +counts=textscan(counts,'%f'); %read them in as floats. +counts=counts{:}; +counts=counts/sum(counts); %normalize the counts into a probability vector +yaux=[0;lambda*counts]; %form the sample vector + + +Aaux=[ones(1,clumns);lambda*trainingmatrix]; %form the k-mer sensing matrix +warning off +xstar=lsqnonneg(Aaux,yaux); %perform the non-negative lease squares +warning on +xstar=xstar/sum(xstar); %return the results as a probability vector \ No newline at end of file diff --git a/src/matlab/quikrTrain.m b/src/matlab/quikrTrain.m new file mode 100644 index 0000000..7960cca --- /dev/null +++ b/src/matlab/quikrTrain.m @@ -0,0 +1,41 @@ +function mat=quikrTrain(inputfasta,k) +%matrix=QuikrTrain(inputfasta,k) Returns the (sparse) k-mer sensing +%matrix from the FASTA file located at 'inputfasta' for the k-mer size k. +%This serves to retrain the Quikr method. The ouput can then be fed to +%quikrCustomTrained(). +%Works for k=1:8 (typically the matrices get too large for k>9) +%The filename for the inputfasta must be the complete path. + +if nargin>2 + error('too many input arguments'); +end + + +[pathtofile,filename,ext]=fileparts(inputfasta); %get the parts of the file + + +outputfilename=fullfile(pathtofile, [filename sprintf('-sensingmatrixK%d.txt',k)]); %Currently this is coded to write a temporary file. In future versions, this will be all be done in RAM +%The reason for writing the file to disk first is that Matlab typically +%crashes when unix() returns as many entries as ./probabilities-by-read +%does (on the order of ~2*10^10). + +kmerfilename=sprintf('%dmers.txt',k); %This contains the list of 6-mers to count. In future versions this will be computed locally instead of being read in. + +if (isunix && not(ismac)) %this is for the linux version + unix(['./probabilities-by-read-linux ' sprintf('%d',k) ' ' inputfasta ' ' kmerfilename ' > ' outputfilename]); %obtain the k-mer counts of the inputfasta read-by-read +elseif ismac %mac version + unix(['./probabilities-by-read-osx ' sprintf('%d',k) ' ' inputfasta ' ' kmerfilename ' > ' outputfilename]); %obtain the k-mer counts of the inputfasta read-by-read +elseif ispc %No PC version + error('Windows is not yet supported'); +end + +fid=fopen(outputfilename); %open the output file + +%A=textscan(fid,'%f'); %get all the counts +%A=A{:}; +A=fscanf(fid,'%f'); +mat=sparse(reshape(A,4^k,length(A)/4^k)); %form into a matrix +mat=bsxfun(@rdivide,mat,sum(mat,1)); %column-normalize +fclose(fid); %close file +delete(outputfilename); %delete the file + diff --git a/src/matlab/quikrWriteOutput.m b/src/matlab/quikrWriteOutput.m new file mode 100644 index 0000000..7dd5c3b --- /dev/null +++ b/src/matlab/quikrWriteOutput.m @@ -0,0 +1,89 @@ +function quikrWriteOutput(directory,filename,xstar,pathtotrainingdatabase,taxonomicrank) +%taxonomic rank: 1=kindom, 2=phylum, 3=class, 4=order, 5=family, +%6=genus, 7=species, 8=strain +%quikrWriteOutput(directory,filename,xstar,trainingdatabase,taxonomicrank) +%will output a Comma Seperated (CSV) file with the file name 'filename' in the directory +%'directory' that gives the summary of concentrations of the reconstruction +%at the taxonomic rank specified by 'taxonomicrank'. The database located +%at'pathtotrainingdatabase' must be in the fasta format with the headers +%in the format output by TaxCollector. +%(see Giongo et al, 2010 TaxCollector: Modifying Current 16S rRNA +%Databases for the Rapid Classification at Six Taxonomic Levels, Diversity. +%doi:10.3390/d2071015). +%The output excel file is suitable for use in visualiztion software such as +%Krona (Ondov, Bergman, and Phillippy, 2011, Interactive Metagenomic +%Visualiztion in a Web Browser, BMC bioinformatics, +%doi:10.1186/1471-2105-12-385). +if nargin>5 + error('too many input arguments. Needs: directory, output filename, xstar, path to training database, and taxonomic rank'); +end +if nargin<5 + error('too few input arguments. Needs: directory, output filename, xstar, path to training database, and taxonomic rank'); +end +[headers,~]=fastaread(pathtotrainingdatabase);%read in the headers of the training database + +for i=1:length(headers) + if not(length(regexp(headers{2},'\[\d\]'))==9) + error(sprintf('The header in entry %d in the training database is not in the Tax Collector format: eg ">[1]kingdom[2]phylum[3]class[4]order[5]family[6]genus[7]species[8]strain|accession"',i)); + end +end + + +seqnames=headers(find(xstar)); %get the names of the sequences that are given nonzero concentration in the reconstruction xstar +basis=find(xstar); %the indicies that have nonzero concentrations + %seqnamesnew=cell(length(basis),1); +for i=1:length(basis) + seqnamesnew{i}=strcat(sprintf('%f[0] ',xstar(basis(i))),seqnames{i}); %format is concentration, and then the name as it is in the training database +end + +for i=1:length(seqnamesnew) + splitup(i,:)=regexp(seqnamesnew{i},'\[\d\]','split'); %split everything up on the token [%d] as per the TaxCollector format +end +%xlswrite(fullfile(directory,[filename '.xls']),splitup); +%so there's a problem: trainset7_112011 is not in the tax collector format. +%So one thing I could do is use the accessions or something like that to +%get the corresponding headers from TaxCollectorRDP10_28 and create a new +%trainset7_112011 (or at least, one with new headers that then work +%according to TaxCollector). + +%So it does look like that for each header in trainset7_112011, after the > +%and before the | that "accession" also appears at the end of some header in +%TaxCollectorRDP10_28 (after the | on the end). So I'd like to make a new +%trainset7_112011 in which the headers are simply replaced. + + +%Now I'm going to try to unique at the given taxanomic rank, and then tally +%up the concentrations of all the corresponding entries in splitup +ranknames=unique(splitup(:,taxonomicrank+2)); %get the unique names at the given taxonomic rank +concentrations=str2double(splitup(:,1)); %pull off the concentrations + +for i=1:length(ranknames) %for each of the names + selectlist=strcmp(splitup(:,taxonomicrank+2),ranknames(i)); %get the rows that have the given name in the proper taxonomic rank + %newsplitup(i,:)={num2str(sum(concentrations(selectlist)),'%f'),ranknames(i)}; %sum up the concentrations in all these rows and put that next to the names just down to the given taxonomic rank + newsplitup(i,:)={num2str(sum(concentrations(selectlist)),'%f'),splitup{find(selectlist,1),3:taxonomicrank+2}}; +end +%xlswrite(fullfile(directory,[filename '.xls']),newsplitup); %write this as the final output + +fid=fopen(fullfile(directory,[filename '.csv']),'w'); +if fid==-1 + error(sprintf('The file %s is currently write-protected (e.g. open or in use in a different program)', fullfile(directory,[filename '.csv']))); +end +[nrows,ncols]=size(newsplitup); +mystr=['%s,']; +for cols=1:ncols-2 + mystr=[mystr ' ' '%s,']; +end +%mystr=[mystr '\n']; +for row=1:nrows + fprintf(fid,[mystr '%s\n'],newsplitup{row,:}); +end +fclose(fid); + + + + + + + + + -- cgit v1.2.3