From 4ca6f92ceb4b2f8c504431cf56f8a6135187a61c Mon Sep 17 00:00:00 2001
From: Calvin <calvin@EESI>
Date: Mon, 11 Mar 2013 12:36:42 -0400
Subject: adding matlab code

---
 src/matlab/Example.m | 52 ++++++++++++++++++++++++++++++++++++++++++++++++++++
 1 file changed, 52 insertions(+)
 create mode 100644 src/matlab/Example.m

(limited to 'src/matlab/Example.m')

diff --git a/src/matlab/Example.m b/src/matlab/Example.m
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+% Here is an example of re-training the quikr method, performing the reconstruction, and outputing the results to a CSV file appropriate for Excel.
+mat=quikrTrain('TaxCollectortrainset7_112011.fa',6); %retrain using the TaxCollector version of trainset7_112011.fa
+xstar=quikrCustomTrained(mat,'testfastafile',6,10000); %perform the reconstruction
+quikrWriteOutput('/path/to/output/director','filenameforoutput',full(xstar),'/path/to/TaxCollectortrainset7_112011.fa',2) %write the output in CSV format, the last argument (2) corresponds to the phylum level. See the file quikrWriteOutput.m for more details.
+
+
+
+
+%This is an example of how to run Quikr
+
+%If you want to use the default training-set (that is, trainset7_112011.fa
+%from RPD), then execute the following
+
+%make sure Matlab/Octave is in the Quikr director
+cd /path/to/Quikr
+
+fastafilename='/path/to/your/fastafile.fasta'; %full path name to your data file
+xstar=quikr(fastafilename); %this will give the predicted reconstruction frequencies
+
+
+%xstar will be on the same basis as trainset7_112011.fa, so to get the
+%sequences that are predicted to be present in your sample:
+[headers,~]=fastaread('trainset7_112011.fa'); %read in the training database
+%note fastaread is not by default included in Octave, the fastaread.m file
+%is included in the Quikr download directory however, and is directly
+%compatible with Matlab.
+nonzeroentries=find(xstar); %get the indicies of the sequences quikr predicts are in your sample
+proportionscell=num2cell(xstar(nonzeroentries)); %convert the concentrations into a cell array
+namescell=headers(nonzeroentries); %Get the names of the sequences
+namesandproportions={namescell{:}; proportionscell{:}}; %This cell array contains the (unsorted) names of the reconstructed sequences and their concentrations (in the first and second columns respectively)
+%so to find which sequence is the most abundant in your mixture:
+[val,ind]=max(xstar(nonzeroentries)); %get the maximum value and it's position
+namesandproportions{1:2,ind} %note that this does not imply this specific strain or species is in your sample, just that phylum/class/order/family/genus this species belongs to is in your sample.
+
+
+% If you would like to use a custom training database, follow the following
+% steps
+fastafilename='/path/to/your/fastafile.fasta'; %full path name to your data file
+trainingdatabasefilename='/path/to/your/trainingdatabase.fasta'; %full path to the FASTA file you wish to use as a training database
+k=6; %pick a k-mer size
+trainingmatrix=quikrTrain(trainingdatabasefilename,k); %this will return the training database
+%then to do the reconstuction
+lambda=10000; %pick a lambda (larger lambda -> theoretically predicted concentrations are closer to actual concentrations), this depends on k-mer size picked, also size and condition of the TrainingMatrix
+xstar=quikrCustomTrained(trainingmatrix,fastafilename,k,lambda); %get the predicted reconstruction frequencies
+
+%again Xstar is on the same basis as the TrainingMatrix, so to get the
+%sequences that are predicted to be present in your sample:
+[headers,~]=fastaread(trainingdatabasefilename); %read in the training database
+nonzeroentries=find(xstar); %get the indicies of the sequences quikr predicts are in your sample
+proportionscell=num2cell(xstar(nonzeroentries)); %convert the concentrations into a cell array
+namescell=headers(nonzeroentries); %Get the names of the sequences
+namesandproportions={namescell{:}; proportionscell{:}}; %This cell array contains the (unsorted) names of the reconstructed sequences and their concentrations (in the first and second columns respectively)
-- 
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