From a36328d0e2980f58cfc36afa2640dfa2f00bb873 Mon Sep 17 00:00:00 2001 From: gailrosen Date: Mon, 12 Aug 2013 16:13:58 -0400 Subject: Update matlab.markdown The other version was not properly viewable. Now it is. --- doc/matlab.markdown | 16 ++++++++++------ 1 file changed, 10 insertions(+), 6 deletions(-) diff --git a/doc/matlab.markdown b/doc/matlab.markdown index ec9249c..09b0103 100644 --- a/doc/matlab.markdown +++ b/doc/matlab.markdown @@ -120,15 +120,15 @@ their concentrations (in the first and second columns respectively) Usage tips: * Please name fasta files of sample reads with .fa<*> and place them - into one directory without any other f ile in that directory (for example, no + into one directory without any other file in that directory (for example, no hidden files that the operating system may generate, are allowed in that - direct ory) + directory) * Note: When making your QIIME Metadata file, the sample id's must match the fasta file prefix names * Fasta files of reads must have a suffix that starts with .fa (e.g.: .fasta and .fa are valid while .fna is NOT) -* Modify the top of the Matlab/Octave scripts for , - , , and +* Modify the top of the Matlab/Octave scripts for \, + \, \, and \ To use with QIIME, one must run the QIIME conversion tool on our OTU table output: @@ -142,13 +142,17 @@ output: Unweighted.) Pre-requisites: -1. +1. \ 2. the tree of the database sequences that were used (e.g. dp7\_mafft.fasttree, gg\_94\_otus\_4feb2011.tre, etc.) -3. your-defined +3. your-defined \ The QIIME procedue: + convert_biom.py -i -o .biom --biom_table_type="otu table" + beta_diversity.py -i .biom -m weighted_unifrac -o beta_div -t (example: rdp7_mafft.fasttree)> + principal_coordinates.py -i beta_div/weighted_unifrac_.txt -o _weighted.txt + make_3d_plots.py -i _weighted.txt -o <3d_pcoa_plotdirectory> -m -- cgit v1.2.3